Simultaneous RP-HPLC Quantification and Characterization of Apigenin-Naringenin Co-Loaded ZnO Nanoparticles for Topical Delivery
DOI:
https://doi.org/10.31489/2959-0663/2-26-3Keywords:
HPLC, polyphenols, simultaneous method, nanoparticles, apigenin, naringenin, isocratic, validationAbstract
Apigenin and Naringenin has proven beneficial prospects owing to an anti-inflammatory, antioxidant and wound healing effects. But having poor water solubility and low bioavailability hinders their application. Therefore, we developed a dual drug-loaded zinc oxide nanoparticle and validated a sensitive and selective liquid chromatography method for simultaneous determination. The nanoparticles were synthesized through chemical precipitation method followed by drug loading. They were evaluated for particle size, surface potential and FTIR, while morphology was determined by scanning electron microscopy. RP-HPLC method utilizing a C18 column as stationary phase and methanol/0.1 % orthophosphoric acid (75:25 v/v) as eluent at a flow rate of 1.0 mL/min with detection at 272 nm. Method was validated as per to ICH Q2(R1) guidelines and having linearity (5–25 µg/mL), accuracy, precision, specificity and robustness. The entrapment efficiencies of Apigenin and Naringenin were 88.6 ± 2.1 % and 85.2 ±1.8 %, respectively. The average size by dynamic-light scattering was 202.67 ± 3.2 nm and zeta potential were –30.51 ± 0.6 mV. Electron-microscopy confirmed mixture of mildly aggregated spherical and rod-shaped particles, size less than 200 nm. The validated analytical method and the nanoparticle system would together serve as a propitious groundwork, for combinatorial delivery of Apigenin and Naringenin.
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Copyright (c) 2026 Ayusha O. Dondulkar, Mandar Muley, Raksha A. Purohit, Nikhil Y. Yenorkar, Natasha S. Akojwar, Satyendra K. Prasad
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
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