Surface-Enhanced Raman Detection of Low-Concentration Methylene Blue Using Nanoparticles Functionalized with 12-Mercaptododecanoic Acid

Authors

  • Indira B. Muslimova Institute of Nuclear Physics ARKAE, Almaty, Kazakhstan
  • Ainur B. Khairusheva L.N. Gumilyov Eurasian National University, Astana, Kazakhstan https://orcid.org/0009-0009-1968-5189
  • Kairat A. Izbassar L.N. Gumilyov Eurasian National University, Astana, Kazakhstan
  • Zhangali A. Bekbol L.N. Gumilyov Eurasian National University, Astana, Kazakhstan
  • Lana I. Lissovskaya Institute of Nuclear Physics ARKAE, Almaty, Kazakhstan; L.N. Gumilyov Eurasian National University, Astana, Kazakhstan
  • Ilya V. Korolkov Institute of Nuclear Physics ARKAE, Almaty, Kazakhstan https://orcid.org/0000-0002-0766-2803

DOI:

https://doi.org/10.31489/2959-0663/3-26-4

Keywords:

SERS, detection, methylene blue, gold nanoparticles, functionalization, 12-mercaptoundecanoic acid, magnetic nanoparticles, Fe3O4, Raman spectroscopy, limit of detection

Abstract

The development of sensitive and magnetically recoverable SERS substrates is of interest for the detection of organic pollutants at low concentrations. In this study, Fe3O4-СА@Au-12-MDA nanoparticles were synthesized for use as a SERS substrate in the detection of methylene blue dye within a concentration range of 10-2-10-7 M. The Fe3O4 magnetic nanoparticles were modified with citric acid, followed by gold deposition and surface functionalization with 12-mercapto-dodecanoic acid. The stepwise modification of the nanoparticles was confirmed by FTIR, SEM-EDX and XRD, which revealed the presence of Au and organic functional groups whilst preserving the crystalline structure of Fe3O4. The Fe3O4-СА@Au-12-MDA-based SERS substrate exhibited signal enhancement compared to conventional Raman spectroscopy. For the characteristic bands at 1623 and 1396 cm-1, the approximate EF values reached 0.11×105 and 0.15×105, respectively, corresponding to 5.95- and 5.40 times signal enhancement. The estimated LOD values calculated using the 3σ criterion were 4.05×10-8 and 5.97×10-8 M for the respective bands. The calibration curves demonstrated good linearity with R2 values of 0.94-0.99 for SERS measurements. The results obtained demonstrate the potential of Fe3O4-СА@Au-12-MDA nanoparticles a magnetic SERS platform for the detection of low concentrations of organic dyes.

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Surface-Enhanced Raman Detection of Low-Concentration Methylene Blue Using Nanoparticles Functionalized with 12-Mercaptododecanoic Acid

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2026-08-28

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Muslimova, I. B., Khairusheva, A. B., Izbassar, K. A., Bekbol, Z. A., Lissovskaya, L. I., & Korolkov, I. V. (2026). Surface-Enhanced Raman Detection of Low-Concentration Methylene Blue Using Nanoparticles Functionalized with 12-Mercaptododecanoic Acid. EURASIAN JOURNAL OF CHEMISTRY, 31(3(123), 53–65. https://doi.org/10.31489/2959-0663/3-26-4

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Vol. 31 No. 3(123) (2026): Eurasian Journal of Chemistry

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PHYSICAL AND ANALYTICAL CHEMISTRY

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Copyright (c) 2026 Indira B. Muslimova, Ainur B. Khairusheva, Kairat A. Izbassar, Zhangali A. Bekbol, Lana I. Lissovskaya, Ilya V. Korolkov

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